rabbit polyclonal antibody against s pneumoniae  (Novus Biologicals)

Journal: Frontiers in Immunology

Article Title: Ethanol Intoxication Impairs Respiratory Function and Bacterial Clearance and Is Associated With Neutrophil Accumulation in the Lung After Streptococcus pneumoniae Infection

doi: 10.3389/fimmu.2022.884719

Figure Lengend Snippet: Effect of ethanol exposure on blood alcohol concentration (BAC) and S. pneumoniae burden in the lung. (A) Schematic diagram of ethanol exposure and S. pneumoniae infection. (B) Representative BAC measurements from serum at 30 minutes post-gavage. Each dot represents the value from a single animal from one experiment. The line for each group represents average value ± SEM. (C) Whole lungs were collected at 24 hours post-infection, homogenized, and plated on agar. S. pneumoniae colony forming units (CFU) were enumerated and data are presented as mean ± SEM. n = 4-6 mice per group per experiment and data are combined from 3 individual experiments. *p < 0.05 by unpaired t test.

Article Snippet: IHC was performed using primary antibodies against Ly6G (1:250, BD Biosciences 551459) and S. pneumoniae (1:1000; Novus Biologicals NB100-64502), and detected using ImmPress polymer reagents (Vector Laboratories).

Techniques: Concentration Assay, Infection

Journal: Frontiers in Immunology

Article Title: Ethanol Intoxication Impairs Respiratory Function and Bacterial Clearance and Is Associated With Neutrophil Accumulation in the Lung After Streptococcus pneumoniae Infection

doi: 10.3389/fimmu.2022.884719

Figure Lengend Snippet: Effect of ethanol exposure on lung inflammation following S. pneumoniae infection. (A) Representative images of lungs from uninfected and infected mice at 24 hours. Top panel = 40x magnification, scale bar = 500 µm. Boxes denote the magnified areas in the middle and bottom panels. Middle and bottom panels = 400x magnification, scale bar = 50 µm; green arrows denote peri-vascular cells and yellow arrows denote airway leukocytes. (B) Quantitative score for criterion of peri-vascular and airway cell accumulation (0-2 score). Data are presented as mean ± SEM. n = 3-6 mice per group per experiment and data are combined from 2 individual experiments. *p < 0.05 by unpaired t test.

Article Snippet: IHC was performed using primary antibodies against Ly6G (1:250, BD Biosciences 551459) and S. pneumoniae (1:1000; Novus Biologicals NB100-64502), and detected using ImmPress polymer reagents (Vector Laboratories).

Techniques: Infection

Journal: Frontiers in Immunology

Article Title: Ethanol Intoxication Impairs Respiratory Function and Bacterial Clearance and Is Associated With Neutrophil Accumulation in the Lung After Streptococcus pneumoniae Infection

doi: 10.3389/fimmu.2022.884719

Figure Lengend Snippet: Effect of ethanol exposure on neutrophil and S. pneumoniae localization and bacterial phagocytosis after infection. (A) IHC staining was performed onformalin-fixed lung sections with antibodies against Ly6G for neutrophils (brown) and S. pneumoniae (pink) and counterstained with hematoxylin. Representative images are at 100x magnification for the top panel (scale bar = 200μm) and 400x magnification for the bottom panel (scale bar = 50 μm). (B) Percent phagocytosis of S. pneumoniae as measured by the ratio of cells with internalized bacteria to total nucleated cells. Black arrows denote cells with internalized S. pneumoniae. Data are presented as mean ± SEM. n = 3-4 mice per group per experiment and are representative of 2 individual experiments. *p < 0.05 by unpaired t test.

Article Snippet: IHC was performed using primary antibodies against Ly6G (1:250, BD Biosciences 551459) and S. pneumoniae (1:1000; Novus Biologicals NB100-64502), and detected using ImmPress polymer reagents (Vector Laboratories).

Techniques: Infection, Immunohistochemistry, Bacteria